COMPARATIVE EVALUATION OF SEED TREATMENTS IN THE TECHNOLOGY STACKING SYSTEM IN SOYBEAN CULTIVATION
DOI:
https://doi.org/10.47820/recima21.v6i11.6891Keywords:
seed treatment, bio-inputs, agricultural managementAbstract
This study evaluated the efficiency of bio-inputs applied via boom sprayer at the V1 stage of soybeans (cultivar MSoy 6403) in a trial conducted in the municipality of Cambé - PR, harvest 2024/25. A randomized block design was adopted, with seven treatments and four replicates, in plots measuring 5.0 m × 5 rows x 0,45 cm between lines (total area of 11,25 m²). The following were measured: stand 20 days after emergence (DAE), dry mass of the aerial part and root weight at 30 DAE, and productivity at 125 DAE. Treatment T6 (Bacillus amyloliquefaciens FZB45 + Bacillus velezensis FZB42 + Trichoderma asperellum kd) resulted in a yield of 4,353.8 ± 112.4 kg ha⁻¹, surpassing the farm's standard treatment (3,589.0 ± 95.1 kg ha⁻¹), representing an increase of 21.3% (Tukey, p < 0.05). The results indicate the agronomic potential of the combined application of microorganisms, suggesting the need for further trials in different environments and economic analysis for large-scale validation.
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ALI, Qurban; KHAN, Abdur Rashid; YUJIE, Wang et al. Antimicrobial metabolites of Bacillus velezensis FZB42 reshape rice rhizosphere microbial community composition and induce host resistance against Rhizoctonia solani. Current Plant Biology, v. 41, p. 100440, 2025, DOI: 10.1016/j.cpb.2025.100440. DOI: https://doi.org/10.1016/j.cpb.2025.100440
BACILIERI, Fernando Simoni; OLIVEIRA, Roberta Camargos De; SANTOS, Ludyellen Cristina Medeiros et al. Soybean cultivars under the foliar application of a compounded biofertilizer in different plant phenological stages and doses. International Journal of Recycling of Organic Waste in Agriculture, v. 12, n. 2, 2023. https://doi.org/10.30486/ijrowa.2022.1946944.1385.
BACKER, Rachel; ROKEM, J. Stefan; ILANGUMARAN, Gayathri et al. Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture. Frontiers in Plant Science, v. 9, n. primeira, 2018. https://doi.org/10.3389/fpls.2018.01473. DOI: https://doi.org/10.3389/fpls.2018.01473
CHAGAS, Lillian França Borges; CHAGAS, Aloísio Freitas; SOARES, Layssah Passos et al. Trichoderma na promoção do crescimento vegetal. Revista de agricultura neotropical, p. 97–102, 2017. Disponível em: https://www.researchgate.net/publication/327785522_TRICHODERMA_NA_PROMOCAO_DO_CRESCIMENTO_VEGETAL. DOI: 10.32404/rean.v4i3.1529 Acesso em: 7 Oct. 2025. DOI: https://doi.org/10.32404/rean.v4i3.1529
CHEN, Qiqi; QIU, Yue; YUAN, Yazhen et al. Biocontrol activity and action mechanism of Bacillus velezensis strain SDTB038 against Fusarium crown and root rot of tomato. Frontiers in Microbiology, v. 13, p. 1–18, 2022. https://doi.org/10.3389/fmicb.2022.994716 DOI: https://doi.org/10.3389/fmicb.2022.994716
CSÓTÓ, András; TÓTH, György; RICZU, Péter; et al. Foliar Spraying with Endophytic Trichoderma Biostimulant Increases Drought Resilience of Maize and Sunflower. Agriculture, v. 14, n. 12, 2024. https//doi.org/10.3390/agriculture14122360 DOI: https://doi.org/10.3390/agriculture14122360
FERREIRA, Lusiane de Sousa. Bacillus amyloliquefaciens: modo de ação e utilizações. Agroadvance, 2025. Disponível em: https://agroadvance.com.br/blog-bacillus-amyloliquefaciens/. Acesso em: 3 oct. 2025.
GENG, Yueyao; CHEN, Shuying; LV, Pinke et al. Positive Role of Trichoderma harzianum in Increasing Plant Tolerance to Abiotic Stresses: A Review. Antioxidants (Basel, Switzerland), v. 14, n. 7, p. 807, 2025. https://doi.org/10.3390/antiox14070807 DOI: https://doi.org/10.3390/antiox14070807
HAN, Xingshan; SHEN, Dongxia; XIONG, Qin et al. The Plant-Beneficial Rhizobacterium Bacillus velezensis FZB42 Controls the Soybean Pathogen Phytophthora. American Society for Microbiology, Applied and environmental microbiology, v. 87, n. 23, p. e0160121, 2021. Disponível em: https://www.researchgate.net/publication/354890997_The_Plant-Beneficial_Rhizobacterium_Bacillus_velezensis_FZB42_Controls_the_Soybean_Pathogen_Phytophthora_sojae_Due_to_Bacilysin_Production, DOI: https://doi.org/10.1128/AEM.01601-21, Acesso em: 3 oct. 2025. DOI: https://doi.org/10.1128/AEM.01601-21
HERMOSA, Rosa; VITERBO, Ada; CHET, Ilan; et al. Plant-beneficial effects of Trichoderma and of its genes. Microbiology, v. 158, n. 1, p. 17–25, 2012. https://doi.org/10.1099/mic.0.052274-0. DOI: https://doi.org/10.1099/mic.0.052274-0
IDRISS, Elsorra E; MAKAREWICZ, Oliwia; FAROUK, Abdelazim; et al. Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect. Microbiology (Reading, England), v. 148, n. Pt 7, p. 2097–2109, 2002. DOI: 10.1099/00221287-148-7-2097. DOI: https://doi.org/10.1099/00221287-148-7-2097
ILLESSAS, M.; MORÁN-DIEZ, M. E.; MARTÍNEZ de Alba, Á. E.; HERMOSA, R.; MONTE, E. Effect of Trichoderma asperellum on wheat plants’ biochemical and molecular responses, and yield under different water stress conditions. International Journal of Molecular Sciences, v. 23, n. 12, art. 6782, 2022. DOI: 10.3390/ijms23126782. DOI: https://doi.org/10.3390/ijms23126782
JUNIOR, Aloísio Freitas Chagas; BORBA, Elias; MARTINS, Albert Lennon Lima et al. Bacillus sp. como promotor de crescimento em soja. Revista de Ciências Agrárias, v. 44, n. 2–3, p. 71–80, 2021.
KHAN, Raja Asad Ali; NAJEEB, Saba; CHEN, Jie et al. Insights into the molecular mechanism of Trichoderma stimulating plant growth and immunity against phytopathogens. Physiologia plantarum, v. 175, n. 6, p. e14133, 2023. DOI: https://doi.org/10.1111/ppl.14133
KONNICKER, Brigitte et al. Combined application of Trichoderma spp. and Bacillus spp. enhances growth and disease resistance in crops: a meta-analysis. Applied Soil Ecology, v. 201, p. 104799, 2025. DOI: https://doi.org/10.1016/j.apsoil.2025.104799
LV, Dongxue; ZHANG, Lei; GUO, Yuanhang et al. Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean. BMC plant biology, v. 25, n. 1, p. 1052, 2025, DOI: 10.1186/s12870-025-07048-x. DOI: https://doi.org/10.1186/s12870-025-07048-x
MACHADO, Daniele Franco Martins; PARZIANELLO, Francini Requia; SILVA, Antonio Carlos Ferreira da; et al. Trichoderma No Brasil: O Fungo E O Bioagente. Revista de Ciências Agrárias, p. 1–26, 2012. Disponível em: https://scielo.pt/pdf/rca/v35n1/v35n1a26.pdf. Acesso em: 7 oct. 2025.
MILLÉO, Marcos Vinícius Ribas; PANDOLFO, Marciele; SANTOS, Danilo Silva dos; et al. Eficiência agronômica de inoculante a base de Bacillus amyloliquefaciens FZB45 para as culturas de milho e soja. Agrária - Revista Brasileira de Ciências Agrárias, v. 18, n. 1, p. e2844–e2844, 2023, https://doi.org/10.5039/agraria.v18i1a2844 DOI: https://doi.org/10.5039/agraria.v18i1a2844
MORETTI, Luiz Gustavo; CRUSCIOL, Carlos Alexandre Costa; LEITE, Marcio Fernandes Alves; et al. Diversos consórcios bacterianos: principais impulsionadores da fertilidade do rizossolo modulando as funções do microbioma, fisiologia vegetal, nutrição e rendimento de grãos de soja. Microbioma ambiental, 2024. Disponível em: https://environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-024-00595-0. Acesso em: 3 Oct. 2025.
NAWAZ, Fahim; RAFEEQ, Rashid; MAJEED, Sadia et al. Biochar Amendment in Combination with Endophytic Bacteria Stimulates Photosynthetic Activity and Antioxidant Enzymes to Improve Soybean Yield Under Drought Stress. Journal of Soil Science and Plant Nutrition, v. 23, n. 1, p. 746–760, 2022. https://doi.org/10.1007/s42729-022-01079-1 DOI: https://doi.org/10.1007/s42729-022-01079-1
NETO, Santiel Alves Vieira; PIRES, Fábio Ribeiro; MENEZES, Carlos et al. Formas de aplicação de inoculante e seus efeitos na cultura da soja. Uberlandia: EDUFU - Editora da Universidade Federal de Uberlandia. Disponível em: https://www.researchgate.net/publication/277171520_Formas_de_aplicacao_de_inoculante_e_seus_efeitos_na_cultura_da_soja. Acesso em: 7 Oct. 2025.
O’CALLAGHAN, Maureen; BALLARD, Ross A.; WRIGHT, David. Soil microbial inoculants for sustainable agriculture: Limitations and opportunities. Soil Use and Management, v. 38, n. 3, p. 1340–1369, 2022, DOI: https://doi.org/10.1111/sum.12811 DOI: https://doi.org/10.1111/sum.12811
POVEDA, Jorge; EUGUI, Daniel. Combined use of Trichoderma and beneficial bacteria (mainly Bacillus and Pseudomonas): Development of microbial synergistic bio-inoculants in sustainable agriculture. Biological Control, v. 176, p. 105100, 2022, DOI: https://doi.org/10.1016/j.biocontrol.2022.105100 DOI: https://doi.org/10.1016/j.biocontrol.2022.105100
REIS, Vanessa M.; ALVES, Bruno J. R.; URQUIAGA, Segundo. Aplicações de inoculantes microbianos na agricultura tropical. Pesquisa Agropecuária Brasileira, v. 58, e02854, 2023. DOI: https://doi.org/10.1590/S1678-3921.pab2023.v58.02854 DOI: https://doi.org/10.1590/s1678-3921.pab2023.v58.02854
SILVA, Mariana Aguiar; NASCENTE, Adriano Stephan; FILIPPI, Marta Cristina Corsi de; et al. Screening of Beneficial Microorganisms to Improve Soybean Growth and Yield. Brazilian Archives of Biology and Technology, 2020, Disponível em: www.scielo.br/babt , http://dx.doi.org/10.1590/1678-4324-2020190463, Acesso em: 6 Oct. 2025. DOI: https://doi.org/10.1590/1678-4324-2020190463
SILVA, R. K. S.; SILVA, P. F. M. Da; NASCIMENTO, L. M. et al. How do rhizobacteria species influence the growth and yield of soybean in a tropical environment? Agronomy Research, 2024. Disponível em: https://dspace.emu.ee/items/aa117ee6-23c8-4df9-8bf2-9dca8bc9cbe2, 21 may 2024, https://doi.org/10.15159/AR.24.032. Acesso em: 6 oct. 2025.
SQUILASSI, Márcio Gomes. Interação de genótipos com ambientes. [S. l.]: Embrapa, 2003. p. 1–47, Disponível em: http://www.cpatc.embrapa.br/publicacoes_2003/Livro_GXE.pdf. Acesso em: 7 oct. 2025.
ZAGO, Lucas Francesquini; CLEBER, Rodrigo; LIMA et al. Inoculação de diferentes doses de Bradyrhizobium por cobertura e seu efeito na cultura da soja. [S. l.]: Universidade Paranaense, 2018. Disponível em: https://www.researchgate.net/publication/329809968_INOCULACAO_DE_DIFERENTES_DOSES_DE_Bradyrhizobium_POR_COBERTURA_E_SEU_EFEITO_NA_CULTURA_DA_SOJA, DOI:10.25110/arqvet.v21i2.2018.7302. Acesso em: 7 Oct. 2025. DOI: https://doi.org/10.25110/arqvet.v21i2.2018.7302
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