EFFECT OF IMBIBITION IN GIBBERELLIC ACID ON THE GERMINATION OF WINGED SEEDS AND SEEDS WITH WINGS REMOVED OF JACARANDA CUSPIDIFOLIA MART
Abstract
Seed germination of Jacaranda cuspidifolia involves interactions between morphological and hormonal factors, and the functional role of seed wings remains poorly understood. This study aimed to evaluate the effects of wing presence and gibberellic acid (GA₃; 10 and 20 mg L⁻¹) on germination. The experiment followed a completely randomized design in a 2 × 3 factorial scheme, with seeds with and without wings subjected to imbibition in water and GA₃ solutions for 24 hours, totaling 2,400 seeds distributed in eight replicates of 50 units. Germination speed index (GSI), mean germination time (MGT), and germination percentage (%G) were evaluated. Data were analyzed using non-parametric tests (Kruskal–Wallis, Dunn, and Mann–Whitney; p ≤ 0.05). Results showed that, in winged seeds, GA₃ did not affect GSI, while MGT was lower in the control and %G increased at 20 mg L⁻¹. In wingless seeds, GSI did not differ, but MGT decreased and %G increased with GA₃, especially at 10 mg L⁻¹. Wing removal increased MGT and reduced germination. It is concluded that the wing plays a functional role in germination, and its removal effects can be partially mitigated by GA₃ application.
Author Biographies
Faculty of Forestry Engineering, Federal University of Mato Grosso, Cuiabá Campus (UFMT).
Agronomist, Federal University of Mato Grosso, Cuiabá Campus (UFMT).
Undergraduate student in Forestry Engineering at the Federal University of Mato Grosso, Cuiabá Campus (UFMT).
Undergraduate student in Forestry Engineering at the Federal University of Mato Grosso, Cuiabá Campus (UFMT).
Faculty of Forestry Engineering, Federal University of Mato Grosso, Cuiabá Campus (UFMT).
Faculty of Forestry Engineering, Federal University of Mato Grosso, Cuiabá Campus (UFMT).
Federal Rural University of Pernambuco (UFRPE).
Departamento de Agricultura, Biodiversidade e Florestas, Universidade Federal de Santa Catarina, Campus de Curitibanos (UFSC).
References
ARRUDA, A. L. A.; SOUZA, D. G.; VIEIRA, C. J. B.; OLIVEIRA, R. F.; PAVAN, F. R.; FUJIMURA, C. Q. L.; RESENDE, U. M.; CASTILHO, R. O. Análise fitoquímica e atividade antimicobacteriana de extratos metanólicos de Jacaranda cuspidifolia Mart. (Bignoniaceae). Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 2, p. 276-281, 2012. DOI: https://doi.org/10.1590/S1516-05722012000200004
BASKIN, C. C., BASKIN, J. M. Seed dormancy and germination ecology in tropical forests. Annual Review of Ecology, Evolution, and Systematics, v. 49, p. 305-331, 2018 DOI: https://doi.org/10.1146/annurev-ecolsys-110617-062639
BASKIN, C. C., BASKIN, J. M. Seed dormancy and germination ecology. Seed Science Research, v. 31, n. 3, p. 171-184, 2021 DOI: https://doi.org/10.1017/S0960258521000125 DOI: https://doi.org/10.1017/S096025852100026X
BOTELHO, R. A; COSTA, P. P; SEMPREBOM, J. P; VIERIA, C. R. Saturação por Bases e Doses de Fósforo (P) no Crescimento Inicial de Mudas de Jacaranda cuspidifolia. UNICIÊNCIAS, v. 28, n. 1, 2024. DOI: https://doi.org/10.17921/1415-5141.2024v28n1p15-19
BRANCALION, P. H. S., et al. Restoration opportunities and challenges in tropical forests. Biological Conservation, v. 238, 108227, 2019 DOI: https://doi.org/10.1016/j.biocon.2019.108227 DOI: https://doi.org/10.1016/j.biocon.2019.108227
CHEN, J.-Z.; HUANG, X.-L.; XIAO, X.-F.; LIU, J.-M.; LIAO, X.-F.; SUN, Q.-W.; PENG, L.; ZHANG, L. Seed dormancy release and germination requirements of Cinnamomum migao, an endangered and rare woody plant in Southwest China. Frontiers in Plant Science, v. 13, art. 770940, 2022. DOI: https://doi.org/10.3389/fpls.2022.770940
COSTA, C. J., DANTAS, B. F. Seed priming and pre-germinative treatments in forest species: physiological responses and limitations. Seed Science Research, v. 31, n. 1, p. 1-12, 2021 DOI: https://doi.org/10.1017/S0960258520000393
FINCH-SAVAGE, W. E.; FOOTITT, S. Seed dormancy cycling and the regulation of dormancy mechanisms to time germination in variable field environments. Journal of Experimental Botany, v. 68, n. 4, p. 843-856, 2017. DOI: https://doi.org/10.1093/jxb/erw477
GENTRY, A. H. Tropical Forest Biodiversity: Distributional Patterns and Their Conservational Significance. Oikos, v. 63, n. 1, p. 19–28, 1992. DOI: https://doi.org/10.2307/3545512
KOŁODZIEJEK, J.; PATYKOWSKI, J.; WALA, M. Effect of light, gibberellic acid and nitrogen source on germination of eight taxa from disappearing European temperate forest, Potentillo albae-Quercetum. Scientific Reports, v. 7, art. 13924, 2017. DOI: https://doi.org/10.1038/s41598-017-13101-z
LORENZI, H. Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 4. ed. Nova Odessa: Instituto Plantarum, 2002.
MAITELLI, G. T. Clima e hidrografia do Estado de Mato Grosso. Revista Mato-Grossense de Geografia, v. 1, p. 25-42, 1994.
MARCHETTI, F. F., CALVI, G. P. Diaspore morphology and germination strategies in tropical tree species. Trees – Structure and Function, v. 36, p. 1431-1444, 2022 DOI: https://doi.org/10.1007/s00468-022-02252-1
NAZIH, A.; BAGHOUR, M.; MAATOUGUI, A.; ABOUKHALID, K.; CHIBOUB, B.; BAZILE, D. Effect of gibberellic acid and mechanical scarification on the germination and seedling stages of Chenopodium quinoa Willd. under salt stress. Plants, [s. l.], v. 13, art. 1330, 2024 DOI: https://doi.org/10.3390/plants13101330 DOI: https://doi.org/10.3390/plants13101330
NEGI, P. S.; SHARMA, S. Effect of different concentrations of gibberellic acid on seed germination behaviour and seedling vigour index of Betula utilis D. Don. Annals of Arid Zone, [s. l.], v. 64, n. 2, p. 199-204, 2025 DOI: https://doi.org/10.56093/aazv64i2.161434 DOI: https://doi.org/10.56093/aaz.v64i2.161434
NONOGAKI, H.; BASSO, M. F.; NAMBARA, E. The molecular and genetic basis of seed dormancy and germination. Plant and Cell Physiology, v. 61, n. 1, p. 39-50, 2020.
RAU, M. et al. Germination physiology and seedling performance of tropical forest species under different hormonal treatments. Forest Ecology and Management, v. 482, 2021.
R CORE TEAM. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing, 2021.
SALARI, H.; KUMAR, S. S.; WANI, O. A.; KUMAR, S.; KUMAR, V. V. Sowing media and gibberellic acid influences germination and seedling growth of chilgoza pine (Pinus gerardiana Wall). International Journal of Plant & Soil Science, v. 35, n. 24, p. 84-99, 2023. DOI: https://doi.org/10.9734/ijpss/2023/v35i244300
SANSON, D.; GARRETT, A. T. de A.; PERES, F. S. B.; BOBROWSKI, R. New understanding of factors influencing the seed germination of Jacaranda micrantha (Cham.), an aesthetically appealing native species. Revista Ceres, Viçosa, v. 71, e71044, 2024 DOI: https://doi.org/10.1590/0034-737X2024710044 DOI: https://doi.org/10.1590/0034-737x2024710044
SANTOS, P. L., SILVA, A. R., COSTA, M. L., OLIVEIRA, F. A. Gibberellic acid enhances seed germination and seedling vigor of tropical tree species. Trees – Structure and Function, v. 33, p. 1203-1215, 2019 DOI: https://doi.org/10.1007/s00468-019-01859-5
SCALON, S. P. Q. Germinação e dormência de sementes florestais. Revista Brasileira de Sementes, v. 28, n. 1, p. 1-12, 2006 DOI: https://doi.org/10.1590/S0101-31222006000100001 DOI: https://doi.org/10.1590/S0101-31222006000100001
SILVA, R. R. P.; MARTINS, S. V.; SILVA, E.; SOUZA, P. B. Seed germination traits and seedling establishment of tropical tree species used in restoration. Forest Ecology and Management, v. 476, 118484, 2020 DOI: https://doi.org/10.1016/j.foreco.2020.118484 DOI: https://doi.org/10.1016/j.foreco.2020.118484
SILVA, M; OLIVEIRA, L. S.; RADAELLI, J. C.; PORTO, A. H.; WAGNER JUNIOR, A. Estratificação e uso de giberelina em sementes de Psidium cattleianum. Pesquisa Aplicada & Agrotecnologia, Guarapuava, v. 11, n. 3, p. 121-125, set./dez. 2018 DOI: https://doi.org/10.5935/PAeT.V11N3.12 DOI: https://doi.org/10.5935/PAeT.V11.N3.12
License
Copyright (c) 2026 RECIMA21 - Revista Científica Multidisciplinar - ISSN 2675-6218
This work is licensed under a Creative Commons Attribution 4.0 International License.
Os direitos autorais dos artigos/resenhas/TCCs publicados pertecem à revista RECIMA21, e seguem o padrão Creative Commons (CC BY 4.0), permitindo a cópia ou reprodução, desde que cite a fonte e respeite os direitos dos autores e contenham menção aos mesmos nos créditos. Toda e qualquer obra publicada na revista, seu conteúdo é de responsabilidade dos autores, cabendo a RECIMA21 apenas ser o veículo de divulgação, seguindo os padrões nacionais e internacionais de publicação.