INFLUENCE OF OZONE THERAPY ON MONONUCLEAR INFILTRATE AND MAST CELLS IN TISSUE REPAIR – IN VIVO PILOT STUDY
Abstract
Objective: To evaluate the effect of ozone gas and ozonized oil on monomorphonuclear infiltrate and mast cell numbers in a standard experimental skin wound healing model. Methods: Thirty Wistar rats received a standardized skin wound and were assigned to three groups: control (CG), ozone gas insufflation (OGG), and topical sunflower ozonized oil (OOG). Euthanasia occurred on days 5 and 10. Histological sections were stained with Hematoxylin-eosin and toluidine blue for quantitative and semi-quantitative analyses. ANOVA followed by Student’s t-test was applied (p<0.05). Results: No significant differences were observed in monomorphonuclear infiltrate among groups at either time point (p>0.05). Mast cell counts, however, were significantly higher in ozone-treated groups, particularly degranulated mast cells on day 5 (OGG p=0.01; OOG p=0.02) compared to controls. Conclusion: Ozone therapy did not significantly modulate monomorphonuclear infiltrate but increased mast cell numbers during early wound healing, regardless of administration route.
Author Biographies
Bacharel em Saúde. Mestre em Processos Interativos dos Órgãos e Sistemas; Instituto de Ciências da Saúde da Universidade Federal da Bahia (ICS, UFBA).
Cirurgiã Dentista pela Escola Bahiana de Medicina e Saúde Pública, Salvador-BA, Brasil.
Mestre em Biotecnologia pelo Programa de Pós-graduação em Biotecnologia. Instituto de Ciências da Saúde. Universidade Federal da Bahia.
Mestre em Medicina e Saúde Humana, Escola Bahiana de Medicina e Saúde Pública, Salvador-BA, Brasil.
Cirurgião bucomaxilofacial. Doutor em Laser pela Universidade Federal da Bahia (UFBA) Escola Bahiana de Medicina e Saúde Pública, Salvado-BA, Brasil.
Professora Associada IV do Departamento de Biointeração do Instituto de Ciências da Saúde. Doutora em Patologia Humana pela FIOCRUZ/UFBA.
References
ALVARENGA, M. O. P. et al. Safety and effectiveness of copaiba oleoresin (C. reticulata Ducke) on inflammation and tissue repair of oral wounds in rats. International Journal of Molecular Sciences, v. 21, n. 10, p. 3568, 2020. DOI: https://doi.org/10.3390/ijms21103568
ATIAKSHIN, D. et al. Mast cells in regeneration of the skin in burn wound with special emphasis on molecular hydrogen effect. Pharmaceuticals, v. 16, n. 3, p. 348, 2023. DOI: https://doi.org/10.3390/ph16030348
ATIAKSHIN, D.; BUCHWALOW, I.; TIEMANN, M. Mast cells and collagen fibrillogenesis. Histochemistry and Cell Biology, v. 154, p. 21–40, 2020. DOI: https://doi.org/10.1007/s00418-020-01875-9
BACCI, S. Fine regulation during wound healing by mast cells: a physiological role not yet clarified. International Journal of Molecular Sciences, v. 23, n. 3, p. 1820, 2022. DOI: https://doi.org/10.3390/ijms23031820
DA SILVA, A. C. et al. Ozonioterapia no tratamento de feridas em cães diabéticos. Brazilian Journal of Development, v. 8, n. 8, p. 56860–56871, 2022. DOI: https://doi.org/10.34117/bjdv8n8-133
DAMY, S. B. et al. Aspectos fundamentais da experimentação animal – aplicações em cirurgia experimental. Revista da Associação Médica Brasileira, v. 56, n. 1, p. 103–111, 2010. DOI: https://doi.org/10.1590/S0104-42302010000100024
DE SIRE, A. et al. Oxygen-ozone therapy for reducing pro-inflammatory cytokines serum levels in musculoskeletal and temporomandibular disorders: a comprehensive review. International Journal of Molecular Sciences, v. 23, n. 5, p. 2528, 2022. DOI: https://doi.org/10.3390/ijms23052528
DONG, J. et al. Mast cells in diabetes and diabetic wound healing. Advances in Therapy, v. 37, p. 4519–4537, 2020. DOI: https://doi.org/10.1007/s12325-020-01499-4
DUNNILL, C. et al. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. International Wound Journal, v. 14, n. 1, p. 89–96, 2017.
EBRAHIMI, P. et al. Effect of photobiomodulation in secondary intention gingival wound healing: a systematic review and meta-analysis. BMC Oral Health, v. 21, p. 258, 2021. DOI: https://doi.org/10.1186/s12903-021-01611-2
EVALUATION of antibacterial activity of sunflower oil: support for nursing. Research, Society and Development, v. 10, n. 9, p. e8710917941, 2021. DOI: https://doi.org/10.33448/rsd-v10i9.17941 Disponível em: https://rsdjournal.org/rsd/article/view/17941
GONZALEZ, A. C. O. et al. Wound healing: a literature review. Anais Brasileiros de Dermatologia, v. 91, n. 5, p. 614–620, 2016. DOI: https://doi.org/10.1590/abd1806-4841.20164741
HUANG, H. J. et al. Effect of ozone water on the inflammation and repair in infected wounds. Nan Fang Yi Ke Da Xue Xue Bao, v. 30, n. 3, p. 515–518, 2010.
LIU, J. et al. Ozone therapy for treating foot ulcers in people with diabetes. Cochrane Database of Systematic Reviews, n. 10, 2015. Art. n. CD008474. DOI: https://doi.org/10.1002/14651858.CD008474.pub2
LIU, L. et al. Ozone therapy for skin diseases: cellular and molecular mechanisms. International Wound Journal, v. 20, n. 6, p. 2376–2385, 2022.
MEDRADO, A. R. et al. Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers in Surgery and Medicine, v. 32, n. 3, p. 239–244, 2003. DOI: https://doi.org/10.1002/lsm.10126
PIIPPONEN, M.; LI, D.; LANDÉN, N. X. The immune functions of keratinocytes in skin wound healing. International Journal of Molecular Sciences, v. 21, n. 22, p. 8790, 2020. DOI: https://doi.org/10.3390/ijms21228790
PIRES, J. R. et al. Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats. Revista de Odontologia da UNESP, v. 50, p. e20210046, 2021. DOI: https://doi.org/10.1590/1807-2577.04621
RODRIGUES, M. et al. Wound healing: a cellular perspective. Physiological Reviews, v. 99, n. 1, p. 665–706, 2019. DOI: https://doi.org/10.1152/physrev.00067.2017
SAGLAM, E. et al. Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats. Journal of Applied Oral Science, v. 28, p. e20190140, 2019. DOI: https://doi.org/10.1590/1678-7757-2019-0140
SHIOTA, N. et al. Pathophysiological role of skin mast cells in wound healing after scald injury: study with mast cell-deficient W/Wv mice. International Archives of Allergy and Immunology, v. 151, p. 80–88, 2009.
SMITH, N. L. et al. Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility. Medical Gas Research, v. 7, n. 3, p. 212–219, 2017.
VIEBAHN-HAENSLER, R.; LEÓN FERNÁNDEZ, O. S. Mitochondrial dysfunction, its oxidative stress-induced pathologies and redox bioregulation through low-dose medical ozone: a systematic review. Molecules, v. 29, n. 12, p. 2738, 2024. DOI: https://doi.org/10.3390/molecules29122738
VINNIK, Y. S. et al. Effectiveness of ozone therapy and ultrasound in regeneration of an infected wound in an experiment. Siberian Medical Review, 2022.
XAVIER, P. B. Application of ozonetherapy in the treatment of skin injuries in the elderly. Research, Society and Development, v. 10, n. 17, p. e229101724682, 2021. DOI: https://doi.org/10.33448/rsd-v10i17.24682 Disponível em: https://rsdjournal.org/rsd/article/view/24682
ZHANG, X. et al. Elastic fibre proteins in elastogenesis and wound healing. International Journal of Molecular Sciences, v. 23, p. 4087, 2022. DOI: https://doi.org/10.3390/ijms23084087
License
Copyright (c) 2026 RECIMA21 - Revista Científica Multidisciplinar - ISSN 2675-6218
This work is licensed under a Creative Commons Attribution 4.0 International License.
Os direitos autorais dos artigos/resenhas/TCCs publicados pertecem à revista RECIMA21, e seguem o padrão Creative Commons (CC BY 4.0), permitindo a cópia ou reprodução, desde que cite a fonte e respeite os direitos dos autores e contenham menção aos mesmos nos créditos. Toda e qualquer obra publicada na revista, seu conteúdo é de responsabilidade dos autores, cabendo a RECIMA21 apenas ser o veículo de divulgação, seguindo os padrões nacionais e internacionais de publicação.