INFLUENCIA DE LA OZONOTERAPIA SOBRE EL INFILTRADO MONONUCLEAR Y LOS MASTOCITOS EN LA REPARACIÓN DE TEJIDOS: ESTUDIO PILOTO IN VIVO
Resumen
Objetivo: Evaluar el efecto del gas ozono y el aceite ozonizado sobre el infiltrado mononuclear y el recuento de mastocitos en un modelo experimental estándar de cicatrización de heridas cutáneas. Métodos: Treinta ratas Wistar se sometieron a un tratamiento estandarizado de heridas cutáneas y se dividieron en tres grupos: control (GC), insuflación de gas ozono (GGO) y aplicación tópica de aceite de girasol ozonizado (GOO). La eutanasia se realizó los días 5 y 10. Las muestras se tiñeron con hematoxilina-eosina y azul de toluidina para análisis cuantitativos y semicuantitativos. Los datos se evaluaron mediante ANOVA seguido de la prueba t de Student (p < 0,05). Resultados: No se observaron diferencias significativas en el infiltrado mononuclear entre los grupos en ninguno de los períodos evaluados (p > 0,05). Sin embargo, los recuentos de mastocitos fueron significativamente mayores en los grupos tratados con ozono, especialmente los mastocitos degranulados en el día 5 (GGO p = 0,01; GOO p = 0,02) en comparación con el grupo control. Conclusión: La ozonoterapia no moduló significativamente el infiltrado mononuclear, pero aumentó el número de mastocitos en la fase temprana de la cicatrización de la herida, independientemente de la vía de administración.
Biografía del autor/a
Licenciatura en Ciencias de la Salud
Maestría en Procesos Interactivos de Órganos y Sistemas; Instituto de Ciencias de la Salud, Universidad Federal de Bahía (ICS, UFBA)
Cirujano Dentista; Facultad de Medicina y Salud Pública de Bahía
Maestría en Biotecnología del Programa de Posgrado en Biotecnología
Instituto de Ciencias de la Salud
Universidad Federal de Bahia
Maestría en Medicina y Salud Humana, Facultad de Medicina y Salud Pública de Bahía
Cirujano Oral y Maxilofacial
Doctorado en Laserterapia por la Universidad Federal de Bahía (UFBA)
Profesor Asociado IV, Departamento de Biointeracción, Instituto de Ciencias de la Salud
Doctorado en Patología Humana por la FIOCRUZ/UFBA
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