HISTOLOGICAL EVALUATION OF SKIN WOUNDS IN RATS SUBJECTED TO PHOTOBIOMODULATION AND OZONE THERAPY
DOI:
https://doi.org/10.47820/recima21.v7i3.7325Keywords:
Wound healing. Low-Level Light therapy. Ozone therapy. Collagen. Inflammation.Abstract
Biomodulatory therapies are becoming increasingly common in healthcare to enhance tissue repair. This study aimed to evaluate and compare the effects of laser photobiomodulation, gaseous ozone, and ozonated oil on tissue repair through histological analysis of skin wounds in rats. Forty Wistar rats were randomly assigned to four groups of 10 animals each: Control Group (CG), Laser Group (LG), Ozone Gas Group (OGG), and Ozonated Oil Group (OOG). Standardized skin wounds were created on the backs of the animals, and the experimental groups received their respective treatments for three consecutive days. Five and ten days post-surgery, five rats from each group were euthanized, and skin samples were collected from the wound area for histological processing. The samples were stained with hematoxylin-eosin and Sirius red. Five standard micrographs of each histological section were analyzed to assess granulation tissue cellularity, collagen organization, and vascular density. A significance level of p<0.05 was established for statistical analysis. On the 10th day after surgery, increased granulation tissue cellularity was observed in all groups except the OGG (p=0.043). A statistically significant difference in collagen organization was noted between the CG and LG on the 5th day (p=0.012). Vascular density was higher in the OOG than in the other experimental groups on the 5th and 10th days (p=0.045 and p=0.004, respectively). In gaseous form, ozone reduced chronic inflammation, while the oil form was particularly noted for its enhanced vascularization during both study periods. The photobiomodulated group demonstrated superior collagen organization.
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