AVALIAÇÃO HISTOLÓGICA DE FERIDAS CUTÂNEAS DE RATOS SUBMETIDAS À FOTOBIOMODULAÇÃO LASER E OZONIOTERAPIA
DOI:
https://doi.org/10.47820/recima21.v7i3.7325Palavras-chave:
Cicatrização, Ozonioterapia, Terapia com LuzResumo
As terapias biomoduladoras tem sido cada vez mais utilizadas na área da saúde para estimular o reparo tecidual. Este estudo objetivou avaliar comparativamente os efeitos da fotobiomodulação laser, ozônio gasoso e óleo ozonizado sobre o reparo tecidual, através da análise histológica de feridas cutâneas de ratos. Quarenta ratos Wistar foram alocados randomicamente em quatro grupos de dez animais cada: Grupo controle (GC), Laser (GL), Gás Ozônio (GGO) e Óleo Ozonizado (GOO). Foram realizados ferimentos cutâneos padronizados no dorso dos animais e os grupos experimentais foram tratados por três dias consecutivos. Cinco e dez dias após a cirurgia, cinco ratos de cada grupo foram eutanasiados e foram coletadas amostras da área da ferida para processamento histológico e coloração com Hematoxilina-eosina e Sírius vermelho. Com cinco micrografias padrão de cada secção histológica, foram avaliadas celularidade do tecido de granulação, expressão do colágeno e densidade vascular. O nível de significância foi de p<0,05. Dez dias após a cirurgia, constatou-se um aumento da celularidade do tecido de granulação em todos os grupos, exceto no GGO (p=0,043). Foi observada uma diferença estatisticamente significativa em relação à organização do colágeno entre o GC e o GL no 5o dia (p=0,012). A densidade vascular foi maior no GOO em relação aos demais grupos experimentais no 5o (p=0,045) e no 10o dia (p=0,004). Na forma de gás, o ozônio atenuou o processo inflamatório crônico, enquanto na forma de óleo se destacou pelo aumento da vascularização nos dois períodos do estudo. O grupo fotobiomodulado apresentou melhor organização do colágeno.
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