ANÁLISE DO TIPO DE DANO CAUSADO POR Croton urucurana EM Candida albicans

Thalita Lisboa Cunha; Paola Souza Manzi; Edson Jose Pereira Junior; Mikaela Aires Martins Ribeiro; João Manoel Palmeira Ferrato Gomes; André Lucas Seixas Macalão; Humberto de Sousa Fontoura; Livia  do Carmo Silva

doi:10.47820/recima21.v4i1.4190

Authors

Thalita Lisboa Cunha https://orcid.org/0000-0002-7729-193X
Paola Souza Manzi https://orcid.org/0000-0003-1380-0181
Edson Jose Pereira Junior Unievangelica
Mikaela Aires Martins Ribeiro https://orcid.org/0000-0002-2704-4770
João Manoel Palmeira Ferrato Gomes https://orcid.org/0000-0001-6642-8702
André Lucas Seixas Macalão https://orcid.org/0000-0002-5263-5900
Humberto de Sousa Fontoura https://orcid.org/0000-0003-4858-1265
Livia do Carmo Silva https://orcid.org/0000-0002-2131-7395

DOI:

https://doi.org/10.47820/recima21.v4i1.4190

Keywords:

Candida albicans. Candidiasis. Croton urucurana. Phytotherapy.

Abstract

Candida albicans is a polymorphic diploid fungi found in normal human microbiota, at times commensal at times oportunistic. Croton urucurana, or ‘'Sangra d’água’’, has therapeutic applications used in medical treatments throughout common cultures, whilst require further developments of its use with efficiency and safety in human conditions. Thus, the current project aims to analyze the action tool of C. urucurana and its outcomes on C. albicans, as well as its cytotoxic activity in human cells. Thereunto, the hydroalcoholic extract of C. urucurana was obtained with an income of 5,24%. From using the colorimetric method with resazurin in a microtiter plate, which has mitocondrial effects, it was obtained a minimum inhibitory concentration (MIC) of 375 mg/mL of the extract, the same found in the presence of ergosterol, a component of the fungal plasmatic membrane. Furthermore, the growth of C. albicans in coexistence with additional sorbitol, an osmotic protector, was tested, raising the minimum inhibitory concentration to 1,500 mg/mL. In order to evaluate the cytotoxicity of C. urucurana, lung fibroblasts were used ensuing in an extract MIC of 375mg/dL. As results, it was found the extract can damage the fungal mitochondrial activity and the cell's wall, although with no deleterious effects on the cell's membrane. Moreover, with the same tested concentrations in fungal cells, it was established its human cells citotoxicity. Thereby, it was figured that C. urucurana has antifungal activity with low selectivity and so is not appliable to testing as a potential antifungal drug.

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