MICROWAVE DISINFECTION OF ACRYLIC RESINS FOR LABORATORY EXPERIMENTAL APPLICATIONS
DOI:
https://doi.org/10.47820/recima21.v3i4.1389Palavras-chave:
acrylic resin specimens can be achievedResumo
Disinfection of acrylic resin specimens can be achieved through microwave irradiation. However, inappropriate settings could lead to physical distortions or impair the decontamination process. This study aims to propose a rapid and reliable microwave disinfection regimen for acrylic resin specimens, in compliance with certified sterility norms, for laboratory experimental applications. Specimens were submitted to monospecific biofilm formation of Staphylococcus aureus, Escherichia coli, and Candida albicans. Thioglycolate fluid medium was used for the qualitative disinfection test during 14 days of incubation at 35° C. Surface roughness, Knoop microhardness, flexural strength, and dimensional stability were also evaluated. Irradiation protocols were 3 or 5 min at 450 W or 650 W in 250 mL of distilled water. Statistical analyses were performed considering α=0.05. All irradiation regimens were able to disinfect the specimens exposed to C. albicans. Only the regimen 450 W for 3 min did not achieve disinfection of the specimens exposed to S. aureus and E.coli. Surface roughness, dimensional stability, and flexural strength were not altered by any regimen (p>0.05). Microhardness decreased in all regimens, except 450 W for 3 min. Irradiation with 450 W for 5 min in 250 mL of water is an optimal disinfection procedure for acrylic resin specimens as it combines optimal potency and exposure time.
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Referências
Al-Saadi, M.H., 2014. Effectiveness of Chemical and Microwave Disinfection on Denture Biofilm Fungi and the Influence of Disinfection on Denture Base Adaptation. J. Indian Prosthodont. Soc. 14, 24–30. https://doi.org/10.1007/s13191-014-0354-2
Aslanimehr, M., Mojarad, N., Ranjbar, S., Aalaei, S., 2018. In vitro comparison of the effects of microwave irradiation and chemical and mechanical methods on the disinfection of complete dentures contaminated with Candida albicans. Dent. Res. J. (Isfahan). 15, 340–346.
Basso, M.F.M., Giampaolo, E.T., MacHado, A.L., Pavarina, A.C., Vergani, C.E., 2012. Evaluation of the occlusion vertical dimension of complete dentures after microwave disinfection. Gerodontology 29, 815–821. https://doi.org/10.1111/j.1741-2358.2011.00567.x
Basso, M.F.M., Giampaolo, E.T., Vergani, C.E., Machado, A.L., Pavarina, A.C., Ribeiro, R.C., 2010. Influence of microwave disinfection on the dimensional stability of denture reline polymers. J. Prosthodont. 19, 364–368. https://doi.org/10.1111/j.1532-849X.2010.00583.x
Campanha, N.H., Pavarina, A.C., Vergani, C.E., Machado, A.L., 2005. Effect of microwave sterilization and water storage on the Vickers hardness of acrylic resin denture teeth. J. Prosthet. Dent. 93, 483–487. https://doi.org/10.1016/j.prosdent.2005.02.016
Code of Federal Regulations, Title 21 Food and Drugs, Part 610.12 Sterility testing. Washington, DC: US Government Printing Office, April 2010., n.d. CFR-2011-title21-vol7-sec610-12.
Consani, R.L.X., Iwasaki, R.Y., Mesquita, M.F., Mendes, W.B., Consani, S., 2008. Effect of repeated simulated disinfections by microwave energy on the complete denture base adaptation. Open Dent. J. 2, 61–66. https://doi.org/10.2174/1874210600802010061
Consant, R.L.X., Vieira, E.B., Mesquita, M.F., Mendes, W.B., Arioli-Filho, J.N., 2008. Effect of microwave disinfection on physical and mechanical properties of acrylic resins. Braz. Dent. J. 19, 348–353. https://doi.org/10.1590/S0103-64402008000400011
Copyright (c) 2011 The United States Pharmacopeial Convention. All rights reserved., n.d. <71>STERILITY TESTS.
Dantas, A., Consani, R., Sardi, J., Mesquita, M., da Silva, M., Sinhoreti, M., 2014. Biofilm formation in denture base acrylic resins and disinfection method using microwave. J. Res. Pract. Dent. 1–9. https://doi.org/10.5171/2014.112424
Dixon, D.L., Breeding, L.C., Faler, T.A., 1999. Microwave disinfection of denture base materials colonized with Candida albicans. J. Prosthet. Dent. 81, 207–214. https://doi.org/10.1016/S0022-3913(99)70250-7
Dovigo, L.N., Pavarina, A.C., Ribeiro, D.G., de Oliveira, J.A., Vergani, C.E., Machado, A.L., 2009. Microwave disinfection of complete dentures contaminated in vitro with selected bacteria. J. Prosthodont. 18, 611–617. https://doi.org/10.1111/j.1532-849X.2009.00489.x
Goiato, M.C., Dos Santos, D.M., Baptista, G.T., Moreno, A., Andreotti, A.M., de Dekon, S.F.C., 2013. Effect of thermal cycling and disinfection on microhardness of acrylic resin denture base. J. Med. Eng. Technol. 37, 203–207. https://doi.org/10.3109/03091902.2013.774444
Gonçalves, A.R., Machado, A.L., Giampaolo, E.T., Pavarina, A.C., Vergani, C.E., 2006. Linear dimensional changes of denture base and hard chair-side reline resins after disinfection. J. Appl. Polym. Sci. 102, 1821–1826. https://doi.org/10.1002/app.24052
Hamouda, I.M., Ahmed, S.A., 2010. Effect of microwave disinfection on mechanical properties of denture base acrylic resin. J. Mech. Behav. Biomed. Mater. 3, 480–487. https://doi.org/10.1016/j.jmbbm.2010.05.002
Izumida, F.E., Ribeiro, R.C., Giampaolo, E.T., Machado, A.L., Pavarina, A.C., Vergani, C.E., 2011. Effect of microwave disinfection on the surface roughness of three denture base resins after tooth brushing. Gerodontology 28, 277–282. https://doi.org/10.1111/j.1741-2358.2010.00393.x
Konchada, J., Karthigeyan, S., Ali, S.A., R, V., Amirisetty, R., Dani, A., 2013. Effect of simulated microwave disinfection on the mechanical properties of three different types of denture base resins. J. Clin. Diagn. Res. 7, 3051–3053. https://doi.org/10.7860/JCDR/2013/7376.3850
Machado, A.L., Breeding, L.C., Vergani, C.E., da Cruz Perez, L.E., 2009. Hardness and surface roughness of reline and denture base acrylic resins after repeated disinfection procedures. J. Prosthet. Dent. 102, 115–122. https://doi.org/10.1016/S0022-3913(09)60120-7
Machado, A.L., Giampaolo, E.T., Pavarina, A.C., Jorge, J.H., Vergani, C.E., 2012. Surface roughness of denture base and reline materials after disinfection by immersion in chlorhexidine or microwave irradiation. Gerodontology 29, e375-82. https://doi.org/10.1111/j.1741-2358.2011.00484.x
Machado, A.L., Giampaolo, E.T., Vergani, C.E., Souza, J.F. de, Jorge, J.H., 2011. Changes in roughness of denture base and reline materials by chemical disinfection or microwave irradiation: Surface roughness of denture base and reline materials. J. Appl. Oral Sci. 19, 521–528. https://doi.org/10.1590/S1678-77572011000500015
Maria Agnese Pinto de Campos, Claudia Kochenborger, Diego Fabris Ferreira da Silva, Eduardo Rolim Teixeira, R.S.A.S., 2009. Effect of repeated microwave disinfection on surface roughness and baseplate adaptation of denture resins polymerized by different techniques. J. Dent. Sci. 24, 40–44.
Mima, E.G., Pavarina, A.C., Neppelenbroek, K.H., Vergani, C.E., Spolidorio, D.M.P., Machado, A.L., 2008. Effect of different exposure times on microwave irradiation on the disinfection of a hard chairside reline resin. J. Prosthodont. 17, 312–317. https://doi.org/10.1111/j.1532-849X.2007.00277.x
Mojarad, N., Khalili, Z., Aalaei, S., 2017. A comparison of the efficacy of mechanical, chemical, and microwave radiation methods in disinfecting complete dentures. Dent. Res. J. (Isfahan). 14, 131–136. https://doi.org/10.4103/1735-3327.205793
Neppelenbroek, K.H., Pavarina, A.C., Spolidorio, D.M.P., Vergani, C.E., Mima, E.G. de O., Machado, A.L., 2003. Effectiveness of microwave sterilization on three hard chairside reline resins. Int. J. Prosthodont. 16, 616–620.
Pavan, S., Arioli Filho, J.N., Santos, P.H. dos, Mollo Jr., F. de A., 2005. Effect of microwave treatments on dimensional accuracy of maxillary acrylic resin denture base. Braz. Dent. J. 16, 119–123. https://doi.org/10.1590/S0103-64402005000200006
Pavarina, A.C., Neppelenbroek, K.H., Guinesi, A.S., Vergani, C.E., Machado, A.L., Giampaolo, E.T., 2005. Effect of microwave disinfection on the flexural strength of hard chairside reline resins. J. Dent. 33, 741–748. https://doi.org/10.1016/j.jdent.2005.02.003
Polychronakis, N., Polyzois, G., Lagouvardos, P., Andreopoulos, A., Ngo, H.C., 2018. Long-term microwaving of denture base materials: effects on dimensional, color and translucency stability. J. Appl. Oral Sci. 26, e20170536. https://doi.org/10.1590/1678-7757-2017-0536
Polychronakis, N., Yannikakis, S., Zissis, A., 2014. The effect of repeated microwaving disinfection on the dimensional stability of acrylic dentures. Acta Stomatol. Croat. 48, 279–284. https://doi.org/10.15644/asc48/4/5
Ribeiro, D.G., Pavarina, A.C., Dovigo, L.N., Palomari Spolidorio, D.M., Giampaolo, E.T., Vergani, C.E., 2009. Denture disinfection by microwave irradiation: a randomized clinical study. J. Dent. 37, 666–672. https://doi.org/10.1016/j.jdent.2009.04.009
Rohrer, M.D., Bulard, R.A., 1985. Microwave sterilization. J. Am. Dent. Assoc. 110, 194–198. https://doi.org/10.14219/jada.archive.1985.0250
Sanitá, P.V., Vergani, C.E., Giampaolo, E.T., Pavarina, A.C., Machado, A.L., 2009. Growth of Candida species on complete dentures: effect of microwave disinfection. Mycoses 52, 154–160. https://doi.org/10.1111/j.1439-0507.2008.01558.x
Sartori, E.A., Schmidt, C.B., Walber, L.F., Shinkai, R.S.A., 2006. Effect of microwave disinfection on denture base adaptation and resin surface roughness. Braz. Dent. J. 17, 195–200. https://doi.org/10.1590/S0103-64402006000300004
Senna, P.M., da Silva, W.J., Cury, A.A.D.B., 2012. Denture disinfection by microwave energy: influence of Candida albicans biofilm. Gerodontology 29, e186-91. https://doi.org/10.1111/j.1741-2358.2010.00439.x
Seo, R.S., Vergani, C.E., Pavarina, A.C., Compagnoni, M.A., Machado, A.L., 2007. Influence of microwave disinfection on the dimensional stability of intact and relined acrylic resin denture bases. J. Prosthet. Dent. 98, 216–223. https://doi.org/10.1016/S0022-3913(07)60058-4
Shafeeq, S.M., Karthikeyan, S., Reddy, S.M., Karthigeyan, S., Manikandan, R., Thangavelu, A., 2016. Cumulative effect of microwave sterilization on the physical properties of microwave polymerized and conventional heat-polymerized acrylic resin. J. Pharm. Bioallied Sci. 8, S100-4. https://doi.org/10.4103/0975-7406.191935
Silva, M., Consani, R., Sardi, J., Mesquita, M., Macêdo, A., Takahashi, J., 2013. Microwave irradiation as an alternative method for disinfection of denture base acrylic resins. Minerva Stomatol. 62, 23–29.
Silva, M.M., Vergani, C.E., Giampaolo, E.T., Neppelenbroek, K.H., Spolidorio, D.M.P., Machado, A.L., McIntyre, F.M., 2006. Effectiveness of microwave irradiation on the disinfection of complete dentures. Int. J. Prosthodont. 19, 288–293. https://doi.org/10.1016/s0084-3717(08)70344-1
Vasconcelos, L.R., Consani, R.L.X., Mesquita, M.F., Sinhoreti, M.A.C., 2013. Effect of chemical and microwave disinfection on the surface microhardness of acrylic resin denture teeth. J. Prosthodont. 22, 298–303. https://doi.org/10.1111/jopr.12009
Wagner, D.A., Pipko, D.J., 2015. The effect of repeated microwave irradiation on the dimensional stability of a specific acrylic denture resin. J. Prosthodont. 24, 25–31. https://doi.org/10.1111/jopr.12203
Xediek Consani, R.L., Chorwat, V., Ferraz Mesquita, M., Fernandes Santos, M.B., Bortolazzo Correr, A., Consani, S., 2014. Effect of simulated microwave disinfection on the linear dimensional change, hardness and impact strength of acrylic resins processed by different polymerization cycles. Minerva Stomatol. 63, 145–154.
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