PROFILE OF POLYPHENOLIC COMPOUNDS EXTRACTED FROM OREGANO (ORIGANUM VULGARE) AT DIFFERENT DECOCTION TIMES

Authors

DOI:

https://doi.org/10.47820/recima21.v5i6.5311

Keywords:

Decoct. ESI-MS. Phytochemistry.

Abstract

Oregano (Origanum vulgare) is an herb used in cooking in dried form as a condiment in food preparation. In traditional medicine this herb is used in the form of teas as a stimulating tonic, to relieve coughs and toothaches, earaches and headaches. It can also be used to treat fever, skin diseases, digestive and respiratory problems. The main secondary metabolites of this species belong to the class of monoterpenes and phenolic compounds, among which there are several biologically active principles, justifying its use as a phytopharmaceutical. To assess the effect of the cooking process on the active principles present in this herb, samples of fresh oregano were collected, dried and subjected to decoction for periods ranging from 5 to 40 minutes. The profile of the extracts obtained was analyzed by Electrospray Ionization Mass Spectrometry (ESI-MS). Analysis of the results showed that the chemical profile of the samples obtained by decoction can be divided into two distinct groups according to time: one group with times of 5, 10 and 20 minutes and the other of 30 and 40 minutes. The results showed that the chemical profile was similar within each of the groups but differed between the groups. The samples belonging to the second group, i.e. those that were subjected to a longer cooking time, showed a great intensification of the molecular mass signals corresponding to the glycosylated flavonoids, making it possible to conclude that increasing the decoction time favours the extraction of these substances whose antioxidant properties are recognized.

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Author Biographies

Augusto Luiz Soares de Lima

Centro Federal de Educação Tecnológica de Minas Gerais – CEFET-MG.

Esther Maria Ferreira Lucas

Centro Federal de Educação Tecnológica de Minas Gerais – CEFET-MG.

Fátima de Cássia Oliveira Gomes

Centro Federal de Educação Tecnológica de Minas Gerais – CEFET-MG.

Ildefonso Binatti

Centro Federal de Educação Tecnológica de Minas Gerais – CEFET-MG.

Marcella Uxa Jacob Werneck Rocha

Centro Federal de Educação Tecnológica de Minas Gerais – CEFET-MG.

References

ABE, L. T.; LAJOLO, F. M.; GENOVESE, M. I. Comparison of Phenol Content and Antioxidant Capacity of Nuts. Ciência e Tecnologia de Alimentos, v. 30, p. 254-259, 2010. DOI: https://doi.org/10.1590/S0101-20612010000500038. DOI: https://doi.org/10.1590/S0101-20612010000500038

BENALI, T. et al. Pharmacological insights into the multifaceted biological properties of quinic acid. Biotechnology & Genetic Engineering Reviews, v. 19, p. 1-30, 2022. DOI: https://doi.org/10.1080/02648725.2022.2122303. DOI: https://doi.org/10.1080/02648725.2022.2122303

BIESAGA, M. Influence of extraction methods on stability of flavonoids. Journal of Chromatography A, v. 1218, n. 18, p. 2505-2512, 2011. DOI: https://doi.org/10.1016/j.chroma.2011.02.059. DOI: https://doi.org/10.1016/j.chroma.2011.02.059

CORDEIRO, S. Z. Origanum vulgare L. Herbário. Rio de Janeiro: Unirio, 2011. https://www.origanumvulgareL. Acessado em: 15 jun. 23.

HRNCIC, M. K. et al. Extraction Techniques and Analytical Methods for Characterization of Active Compounds in Origanum Species. Molecules, v. 25, p. 4735, 2020. DOI: https://doi.org/10.3390/molecules25204735. DOI: https://doi.org/10.3390/molecules25204735

IRAKLI, M. et al. LC-MS Identification and Quantification of Phenolic Compounds in Solid Residues from the Essential Oil Industry. Antioxidants, v. 10, n. 12, p. 2016, 2021. DOI: https://doi.org/10.3390/antiox10122016. DOI: https://doi.org/10.3390/antiox10122016

MA, L.; TANG, L.; YI, Q. Salvianolic Acids: Potential Source of Natural Drugs for the Treatment of Fibrosis Disease and Cancer. Front Pharmacology, v. 10, p. 97, 2019. DOI: https://doi.org/10.3389/fphar.2019.00097. DOI: https://doi.org/10.3389/fphar.2019.00097

MENEZES, J. C. J. et al. Volatile compounds and quality analysis in commercial medicinal plants of Camellia sinensis. Ciência Rural, v. 49, n. 03, p. e20180548, 2019. DOI: https://doi.org/10.1590/0103-8478cr20180548. DOI: https://doi.org/10.1590/0103-8478cr20180548

NASCIMENTO, Y. E. Influência da natureza do solvente e do aquecimento na extração de terpenos bioativos de sementes de sucupira branca (Pterodon emarginatus). In: XXX Encontro Regional da Sociedade Brasileira de Química, 2016.

PEZZANI, R.; VITALINI, S.; IRITI, M. Bioactivities of Origanum vulgare L.: an update. Phytochemistry Reviews, v. 16, p. 1253-1268, 2017. DOI: https://doi.org/10.1007/s11101-0179535-z. DOI: https://doi.org/10.1007/s11101-017-9535-z

QIN, T. et al. Salvianolic acid A & B: potential cytotoxic polyphenols in battle against cancer via targeting multiple signaling pathways. International Journal of Biological Science, v. 15, n. 10, p. 2256–2264, 2019. DOI: doi: https://doi.org/10.7150/ijbs.37467. DOI: https://doi.org/10.7150/ijbs.37467

SILVA, J. P. L. et al. Oregano essential oil: influence of the chemical composition on the inhibitory activity against Salmonella enteritidis. Ciência & Tecnologia de Alimentos, v. 30, p. 136-141, 2010. DOI: https://doi.org/10.1590/S0101-20612010000500021. DOI: https://doi.org/10.1590/S0101-20612010000500021

SIMÕES, C. M. O. Farmacognosia – da Planta ao Medicamento. Florianopolis: Editora da UFSC, 2000.

SINGH, P.; KOTHIYAL, P.; RATAN, P. Pharmacological and phytochemical studies of Origanum vulgare: a review. International Research Journal of Pharmacy, [S. l.], v. 9, n. 6, p. 30-35, 2018. DOI: https://doi.org/10.7897/2230-8407.09685. DOI: https://doi.org/10.7897/2230-8407.09685

SOLTANI, S. et al. Review of the Phytochemistry and Antimicrobial Properties of Origanum vulgare L. and Subspecies. Iranian Journal of Pharmaceutical Research, v. 20, n. 2, p. 268-285, 2021. DOI: https://doi.org/10.22037/ijpr.2020.113874.14539.

YI LEI NGO, Y. L.; LAU, C. H.; CHUA, L. S. Review on rosmarinic acid extraction, fractionation and its anti-diabetic potential. Food and Chemical Toxicology, v. 121, p. 687-700, 2018. DOI: https://doi.org/10.1016/j.fct.2018.09.064. DOI: https://doi.org/10.1016/j.fct.2018.09.064

ZEJLI, H. et al. Phytochemical analysis, antioxidant, anti-inflamatory, hemagglutinin and hemolytic activities of chemically characterized extracts from Origanum grossi (L.) and Thymus pallidus (L.). Plants, v. 13, p. 385, 2024. DOI: https://doi.org/10.3390/plants13030385. DOI: https://doi.org/10.3390/plants13030385

Published

05/06/2024

How to Cite

Soares de Lima, A. L., Ferreira Lucas, E. M., Oliveira Gomes, F. de C., Binatti, I., & Uxa Jacob Werneck Rocha, M. (2024). PROFILE OF POLYPHENOLIC COMPOUNDS EXTRACTED FROM OREGANO (ORIGANUM VULGARE) AT DIFFERENT DECOCTION TIMES. RECIMA21 - Revista Científica Multidisciplinar - ISSN 2675-6218, 5(6), e565311. https://doi.org/10.47820/recima21.v5i6.5311