DESINFECCIÓN POR MICROONDAS DE RESINAS ACRÍLICAS PARA APLICACIONES EXPERIMENTALES DE LABORATORIO
DOI:
https://doi.org/10.47820/recima21.v3i4.1389Palabras clave:
desinfección de las muestras de resinaResumen
La desinfección de las muestras de resina acrílica se puede lograr a través de la irradiación de microondas. Sin embargo, la configuración inadecuada podría provocar distorsiones físicas o perjudicar el proceso de descontaminación. Este estudio tiene como objetivo proponer un régimen de desinfección por microondas rápido y confiable para muestras de resina acrílica, de acuerdo con las normas de esterilidad certificadas, para aplicaciones experimentales de laboratorio. Los especímenes fueron sometidos a la formación de biopelículas monoespecíficas de Staphylococcus aureus, Escherichia coli y Candida albicans. Se utilizó medio fluido de tioglicolato para la prueba de desinfección cualitativa durante 14 días de incubación a 35 ° C. También se evaluaron la rugosidad de la superficie, la microdureza de Knoop, la resistencia a la flexión y la estabilidad dimensional. Los protocolos de irradiación fueron de 3 o 5 min a 450 W o 650 W en 250 mL de agua destilada. Se realizaron análisis estadísticos considerando α=0,05. Todos los regímenes de irradiación pudieron desinfectar las muestras expuestas a C. albicans. Sólo el régimen de 450 W durante 3 min no logró la desinfección de las muestras expuestas a S. aureus y E.coli. La rugosidad de la superficie, la estabilidad dimensional y la resistencia a la flexión no se vieron alteradas por ningún régimen (p>0,05). La microdureza disminuyó en todos los regímenes, excepto 450 W durante 3 min. La irradiación con 450 W durante 5 min en 250 mL de agua es un procedimiento de desinfección óptimo para muestras de resina acrílica.
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